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In vitro assays

Pharmatest's in vitro cancer cell assays include assays of cell viability and cell invasion. Cell viability is measured using CellTiter-Glo® luminescent cell viability assay (CTG), a homogeneous method for determining the number of viable cells in culture. The cell viability assays are rapid and reliable screening tools for cancer drug discovery, and they can be combined with live cell imaging using IncuCyte.

Cancer cell invasion is measured using a commercially available BD BioCoatTM FluoroBlokTM Invasion System, where the cancer cells are grown on a basement membrane-like MatrigelTM coated filter chamber. The capacity of the cells to migrate through the MatrigelTM layer is determined and cell invasion is quantitated by post-labelling the cancer cells with a fluorescent dye. The subsequent fluorescence of the invaded cells is then measured as endpoint data.